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KMID : 0380420160400030109
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Journal of Prventive Veterinary Medicine
2016 Volume.40 No. 3 p.109 ~ p.114
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Analysis of Choline in infant formula with Ion Chromatography in Korean domestic market
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Park Ji-Sung
Ryu Eun-Chae
Park Jae-Woo
Kim Hyun-Soo
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Abstract
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Choline is an amine essential to maintenance of good health. Here, we modified the ion chromatographic method for measuring choline described by Laikhtman & Rohrer and validated by the ICH guideline and applied it to monitoring of choline content in infant formula in the Republic of Korea. The Certified Reference Materials (CRM), NIST 1849a, were used for validation of the modified method. The parameters of validation were linearity, range, specificity, accuracy, precision, LOD, LOQ and recovery. The linearity, R², was above 0.999 and the analytical range was 2.5 to 50 μg/mL. The specificity was confirmed by the retention time (RT) and positive tests with calibration standard and CRM. The accuracy, precision and recovery values were 99.6 ± 2.41 %, 2.42 % and 90.8 - 92.8 %. The LOD and LOQ values were 0.03 mg/kg and 0.17 mg/kg, respectively. Five livestock products inspection agencies voluntarily participated in the collaborative study. The result, HORRAT value was 0.8 which was within the reference value 0.5 - 2.0. The validated method was applied to the analysis of choline in infant formula in the Republic of Korea. The results also conformed to ``The Standard for Livestock Products Labeling``.
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KEYWORD
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Choline, infant formula, ion chromatography, validation
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